Qualification Level: TESDA NC I – Sustainable Resin Induction
Crop Focus: Agarwood (Aquilaria spp.)
Objective: Apply inoculation at the right time using safe, standardized field protocols to maximize resin formation
I. INTRODUCTION
Resin formation in Agarwood is stress-induced, and inoculation must be carefully timed and executed to ensure tree health, effective microbial colonization, and high-quality resin.
Proper field protocols prevent infections, tree death, and microbial contamination.
II. TIMING OF INOCULATION
| Factor | Best Timing | Notes |
|---|---|---|
| Tree age | 3–5 years (young to mature trees) | Seedlings and very young trees are vulnerable; mature trees tolerate controlled stress better |
| Season / climate | End of dry season to early rainy season | Moderate soil moisture favors microbial establishment; avoid waterlogged conditions |
| Tree health | Healthy, well-fertilized, disease-free trees | Trees under stress from drought or pests have reduced resin potential |
| Soil condition | Moist, well-drained, enriched with biofertilizers | Pre-inoculation irrigation may be applied 1–2 days before injection to optimize microbial activity |
III. FIELD PROTOCOLS
1. PRE-INOCULATION PREPARATION
- Select target trees and mark them
- Record tree ID, age, and health status
- Prepare inoculant (BarIno FusaTrinity™, Mycoboost, or agar plug)
- Check soil moisture; irrigate lightly if too dry
- Sanitize tools (drills, knives, syringes) to prevent contamination
2. WOUND OR HOLE PREPARATION
- Drill or cut small, clean wounds at target height
- Avoid excessive depth to prevent tree stress or structural damage
- Ensure wounds are spaced to allow resin formation without compromising tree stability
3. INOCULANT APPLICATION
- Insert inoculant into wound (liquid, paste, or agar plug)
- Apply biofertilizers or microbial enhancers around root zone to support tree defense
- Seal wound with wax, biodegradable plug, or clay to prevent contamination
4. POST-INOCULATION CARE
- Monitor tree moisture; avoid waterlogging
- Mulch around base to conserve moisture and enhance microbial activity
- Inspect trees regularly for excessive stress, pests, or infections
- Record date, method, inoculant type, and tree response
IV. SAFETY AND SANITATION PROTOCOLS
- Wear gloves and protective clothing
- Disinfect tools between trees to avoid cross-contamination
- Handle fungal inoculants carefully; avoid inhalation
- Wash hands after field operations
V. MONITORING SCHEDULE
| Post-Inoculation Stage | Frequency | Observation |
|---|---|---|
| First 2 weeks | Twice a week | Wound condition, microbial colonization, moisture |
| 1–3 months | Weekly | Tree stress, resin initiation, pest incidence |
| 3–12 months | Monthly | Resin development, tree health, fungal activity |
VI. BEST PRACTICES
- Always use healthy, well-nourished trees
- Apply inoculants during moderate soil moisture conditions
- Use minimum wounding necessary to induce resin
- Combine with biofertilizers and organic amendments for optimal microbial activity
- Maintain record-keeping for traceability and evaluation
VII. TESDA NC I PERFORMANCE STANDARD
Learner must demonstrate ability to:
- Select the right tree age and season for inoculation
- Follow field protocols safely and effectively
- Apply inoculants correctly using chosen system (hole, syringe, paste, frill, agar plug)
- Monitor tree response and maintain records
VIII. FARMER-FRIENDLY CHECKLIST
☐ Trees healthy, disease-free, and appropriate age
☐ Soil moisture checked; pre-irrigation applied if necessary
☐ Tools and inoculants prepared and sanitized
☐ Wounds or holes made carefully without excessive damage
☐ Inoculant applied and sealed properly
☐ Mulch applied around base
☐ Post-inoculation monitoring scheduled and records updated
Key Takeaway: Following correct timing and field protocols ensures controlled, safe resin induction, improves resin quality, and integrates smoothly with biofertilizer use, irrigation, and organic soil management.